the gout disease symptoms:
pain in the feet (usually at the corm of the foot) increases after wake up .
pain in the large finger of the foot.
Note:
if the patient have this symptoms and the UA analysis result is normal he must take the drug of gout disease.
Procedure:
1. Put 1 ml of the uric acid reagent in each of two small test tubes.
2. Withdraw 20µ of serum by micropipette and put them in one of the two tubes and sign it as test tube .
3. Withdraw 20µ of uric acid standard and put them in the other tube and sign it as standard tube .
4. Leave the two tubes in water bath for 5 minutes or in room temperature for 10 minutes .
5. Use the wave length 546 nm for measuring.
6. Put the standard and record the reading then put the tested serum and record the reading.
Calculation:
[The reading of test / The reading of standard ] * conc. of UA standard (usually 6 or 5 mg/dl) .
Normal values:
♂ : 3.4 – 7.0 mg/dl
♀ : 2.4 – 5.7 mg/dl
Nov 22, 2010
Creatinine analysis
Procedure:
-In Blood
Sample serum or plasma
The reagent usually must be prepared before use according to kits of the chemicals.
1.put 1 ml of working reagent in two test tubes.
2.put 100 µ of serum in one tube and read after exact 30 sec. abs1 at wave length 500 then wait for additional 2 minutes and read again abs2.
3.put 100 µ of standard in the other tube read after exact 30 sec. abs1 then wait for additional 2 minutes and read again abs2.
Calculation:
abs of sample (abs2 - abs1) / abs of standard (abs2 - abs1) * 2 = .......mg / dl
-In urine
the urine must be collected for 24 hrs. then take 1ml urine and put on it 49 ml water then take from the solution 100 µ and repeat as shown above
Calculation:
abs of sample / abs of standard * 100 = ....... mg / dl.
Normal values
in serum or plasma 0.6 - 1.4 mg / dl
in urine 1 - 1.5 mg / dl
-In Blood
Sample serum or plasma
The reagent usually must be prepared before use according to kits of the chemicals.
1.put 1 ml of working reagent in two test tubes.
2.put 100 µ of serum in one tube and read after exact 30 sec. abs1 at wave length 500 then wait for additional 2 minutes and read again abs2.
3.put 100 µ of standard in the other tube read after exact 30 sec. abs1 then wait for additional 2 minutes and read again abs2.
Calculation:
abs of sample (abs2 - abs1) / abs of standard (abs2 - abs1) * 2 = .......mg / dl
-In urine
the urine must be collected for 24 hrs. then take 1ml urine and put on it 49 ml water then take from the solution 100 µ and repeat as shown above
Calculation:
abs of sample / abs of standard * 100 = ....... mg / dl.
Normal values
in serum or plasma 0.6 - 1.4 mg / dl
in urine 1 - 1.5 mg / dl
Urea
Procedure :
1.prepare two test tubes mark one as test tube and the other is standard tube .
2.in standard tube put : 1 ml buffer + one drop urease (R2) + 10 µ standard reagent (R1) leave for 5 min. in room temp.
3.in test tube put at same time : 1 ml buffer + one drop urease (R2) + 10 µ serum or plasma then leave for 5 min in room temp.
4.then put 200 µ of Reagent 4 (R4) and leave for 5 min. in water bath or 10 min. in room temp.
5.Measure at 580 wave length.
Calculation :
[ reading test / reading standard ] x 50 =.......mg / dl . OR [ reading test / reading standard ] x 8.33 =......mmol /l
Normal values:
15 - 45 mg / dl.
OR
2.5 - 7.5 mmol / l.
1.prepare two test tubes mark one as test tube and the other is standard tube .
2.in standard tube put : 1 ml buffer + one drop urease (R2) + 10 µ standard reagent (R1) leave for 5 min. in room temp.
3.in test tube put at same time : 1 ml buffer + one drop urease (R2) + 10 µ serum or plasma then leave for 5 min in room temp.
4.then put 200 µ of Reagent 4 (R4) and leave for 5 min. in water bath or 10 min. in room temp.
5.Measure at 580 wave length.
Calculation :
[ reading test / reading standard ] x 50 =.......mg / dl . OR [ reading test / reading standard ] x 8.33 =......mmol /l
Normal values:
15 - 45 mg / dl.
OR
2.5 - 7.5 mmol / l.
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